ITT5746
ITT5746
- Catalog: ITT5746
- Gene/Protein: TLE1/2/3/4
- Product Description: Immunotag™ TLE1/2/3/4 Polyclonal Antibody
385.0000
Price in reward points: 385
Your shopping cart is empty!
Antibody Specification | |
Datasheet | |
Target Protein | TLE1/2/3/4 |
Clonality | Polyclonal |
Storage/Stability | -20°C/1 year |
Application | WB,ELISA |
Recommended Dilution | WB: 1:500-2000, EIA: 1:10000-20000 |
Concentration | 1 mg/ml |
Reactive Species | Human,Mouse,Rat |
Host Species | Rabbit |
Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human TLE1/TLE2/TLE3/TLE4. AA range:721-770 |
Specificity | TLE1/2/3/4 Polyclonal Antibody detects endogenous levels of TLE1/2/3/4 |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
Gene Name | TLE1/2/3/4 |
Accession No. | Q04724/Q04725/Q04726/Q04727 |
Alternate Names | similar to transducin-like enhancer of split 1/2/3/4 |
Description | function:Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.,PTM:Phosphorylated, probably by CDC2. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.,similarity:Belongs to the WD repeat Groucho/TLE family.,similarity:Contains 6 WD repeats.,subcellular location:Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.,subunit:Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif).,tissue specificity:In all tissues examined, mostly in brain, liver and muscle., |
Protein Expression | Aorta endothelial cell,Colon,Fetal brain,Kidney, |
Subcellular Localization | nucleus,nucleoplasm,nucleolus,cytoplasm,cytosol, |
Protein Function | function:Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.,PTM:Phosphorylated, probably by CDC2. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.,similarity:Belongs to the WD repeat Groucho/TLE family.,similarity:Contains 6 WD repeats.,subcellular location:Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.,subunit:Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif).,tissue specificity:In all tissues examined, mostly in brain, liver and muscle., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |