A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilized on a chromatographic resin.
The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction.
In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilized in research laboratories.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
- Teaches antibody:antigen precipitation.
- Hands-on activity to precipitate antigen and antibody complex.
- Quantitative assessment of antibody concentration.