5-alpha Chemically Competent Cells

G-Biosciences 5-alpha Chemically Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. Chemically competent 5-alpha E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Features:

• Clone large plasmids and BACs

• Reduced recombination of cloned DNA (recA1)

• Suitable for blue/white screening by α-complementation of the β-galactosidase gene

• Resistance to phage T1 (fhuA)

• Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)

Genotype:

fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Transformation efficiency:  1 x 10⁹ cfu/µg pUC19 DNA