BL21 (DE3) Chemically Competent Cells

G-bio BL21 (DE3) chemical competent cell is a widely used E. coli strain. It is suitable for protein expression under T7 promoter.

Features

•  T7 Expression Strain

•  Deficient in proteases Lon and OmpT

•  Resistance to phage T1 (fhuA)

•  Free of animal products)

Genotype

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5

Transformation efficiency:  1-2 x 10⁷ cfu/µg pUC19 DNA