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XIT™ Genomic DNA from Tissue (1 Citations)

The XIT™ Genomic DNA from Tissue kit is designed for the isolation of genomic DNA from fresh, frozen or methanol/ acetone fixed tissues.
 
The kit uses three main steps (see figure) that are rapid & efficient cell lysis, enzymatic protein digestion and complete protein precipitation, followed by DNA precipitation.  This yields high quality genomic DNA.
 
The kit is supplied with three protocols for the isolation of:
  1. 1-10mg fresh or frozen tissue
  2. 50-100mg fresh or frozen tissue
  3. 5-10mg fixed tissue 
 
Three sizes of kit are available for processing a total of 0.25, 2.5 and 10g of tissue.  The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in size.  The yield is approximately 0.5-10µg/mg tissue.
 

 


Features

 

 

 

  • Isolate high quality genomic DNA
  • Suitable for fresh, frozen and fixed tissue
  • Yield 0.5-10µg/mg tissue
  • Suitable for archive quality DNA.

 

 

Protocol
786-345
786-346
786-347
Material Safety Data Sheet
786-345
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786-346
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-347
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Molecular Biology Handbook  A guide to our products for DNA and RNA.
Certificate Of Analysis
786-346
180712

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