Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast PE LB™ is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast- PE LB™ (see Related Products for protease inhibitor Protease Arrest™). The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells. The kit is good for 100 preps of 50µl yeast cell pellet.
APPLICATIONS
Preparation of yeast spheroplast and extraction of yeast proteins. This kit is suitable for processing approximately 10ml yeast cell pellet suspension, either single or multiple smaller preps.
COMPATIBILITY
Yeast PE LB™ is compatible with any downstream application including running various chromatography procedures and gel electrophoresis applications. Yeast PE LB™ is also compatible for protein estimation with NI™ protein assay.
Features
- Eliminates the need for glass bead lysis.
- Supplied as a kit, containing Zymolyase® and reagents needed for protein extraction.
- Suitable for processing 100 x 50µl yeast cell pellet preparations.
Applications
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Lysis and extraction of proteins from yeast cells.
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Isolation of spheroplasts.
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For the isolation of biologically active proteins.
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Suitable for most applications including enzyme and protein purifications, electrophoresis, Western blotting and 2D-gel analysis.
