Bacterial transformation allows researchers to insert their recombinant DNA into bacteria, which then multiply making more copies of the transformed bacteria. The transformed plasmid can also be used by the bacteria as a template to make recombinant protein.
Students will explore the principles of bacterial transformation and will learn how to make bacteria susceptible, or competent, for transformation. They will also transform bacteria with DNA that confers antibiotic resistance and encodes for a light emitting protein. The results of the transformation can be easily visualized under UV light as glowing bacteria.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
- Preparation of Competent cells.
- Bacterial transformation.
- Antibiotic selection of transformed bacteria.