FOCUS™ Membrane Proteins (10 Citations)
Catalog
Description
Size
Price(USD)
Qty
Catalog
786-249
786-249
Description
FOCUS™ Membrane Proteins
FOCUS™ Membrane Proteins
Size
50 Preps
50 Preps
$451.00
$451.00
FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane proteins are extracted with a single step phase partition, with an efficiency greater than 90% with minimal cross-contamination from hydrophilic proteins.
The kit is provided with reagents necessary for extraction of membrane proteins and their subsequent preparation for isoelectric focusing or 2D gel resolution for improved spot resolution.
FOCUS™ Membrane Proteins kit is designed for >50 preps, where 1 prep is:
- 100mg mammalian tissue
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50µl wet animal cell pellet
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50µl wet yeast pellet
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50µl wet bacteria pellet
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250mg plant tissue
Features
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Phase partition based extraction of membrane proteins.
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Fractions suitable for 2D electrophoresis and other applications.
Applications
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Selective fractionation of membrane proteins from tissues, cells, plants, yeast, bacteria, insects, and other sources.
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Suitable for membrane protein preparation for 2D gel electrophoresis and other applications.
Protocol | |
786-249 |
Material Safety Data Sheet | |
786-249 |
Technical Literature | |
Mass Spectrometry Sample Prep Handbook | A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation. |
Plant Proteomics Handbook | |
Protein Electrophoresis Handbook | A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products. |
Sample Preparation Handbook | For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment |
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- Weam W. Ibrahim, Krystyna Skalicka-Woźniak, Barbara Budzyńska, Nesrine S. El Sayed, NLRP3 inflammasome inhibition and M1-to-M2 microglial polarization shifting via scoparone-inhibited TLR4 axis in ovariectomy/D-galactose Alzheimer's disease rat model, International Immunopharmacology, Volume 119, 2023, 110239, ISSN 1567-5769, https://doi.org/10.1016/j.intimp.2023.110239
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- Lu, Che-Wei et al (2022) Bioremediation potential of cadmium by recombinant Escherichia coli surface expressing metallothionein MTT5 from Tetraymena thermophila. CHEMOSPHERE. https://doi.org/10.1016/j.chemosphere.2022.136850
- Manzer, Haider S. et al (2022) The group B streptococcal adhesin BspC interacts with host cytokeratin 19 to promote colonization of the female reproductive tract. MBIO. https://doi.org/10.1128/mbio.01781-22
- Rodriquez-Vazquez, Mariana et al (2021) Overexpression and extra-mitochondrial localization of the chaperonin Hsp60 in ameloblastoma. J ORAL BIOSCIENCES. https://doi.org/10.1016/j.job.2021.05.001
- De los Santos, M. et al (2019) Cellular Retention Assay to Determine the Interaction Frequency of CD19-directed Chimeric Antigen Receptor (CAR) Engineered Cells against CD19+ Leukemic Cells. Bio-101:e3358
- Bhat, R. et al (2015) J. Agric. Food Chem. DOI: 10.1021/acs.jafc.5b04468
- Zhao, S. et al (2014) J. Proteomicss. 101:102
- Wang, J. et al (2013) Appl. Microniol. Biotech. 97:2077