FOCUS™ SubCell Kit (15 Citations)
FOCUS™ SubCell is designed for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondrial, cytosolic, and membrane proteins. The resulting mitochondrial fraction can be subsequently separated into heavy and light fractions by gradient centrifugation and the resulting mitochondria are >90% active and have intact inner and outer membranes. The kit includes reagents for optional steps that minimize contaminations of the nuclear fraction by cytoplasmic elements.
FOCUS™ SubCell is suitable for cultured animal cells and can be adapted for animal tissues. The kit is designed for 50 preps, where one prep is equivalent to 2x107 mammalian cells or 100mg mammalian tissue.
- Adaptable for animal tissue
- Resulting fractions suitable for 2D electrophoresis and other applications
- Isolated mitochondria are ~90% active
Fractionation of cells and tissues into mitochondrial, nuclear, cytosolic and membrane proteins
Suitable for proteomic and cell signalling studies
|Material Safety Data Sheet|
|Enrichment of Nuclear, Mitochondrial, Cytosolic and Membrane Proteins with FOCUSâ„¢ SubCell|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|
|Plant Proteomics Handbook|
|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|
|Sample Preparation Handbook||For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment|