The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult human hearts.
The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.
Blot lane order:
- Protein marker
- Human Whole Heart Lysate
- Human Left Atrium Lysate
- Human Right Atrium Lysate
- Human Left Ventricle Lysate
- Human Right Ventricle Lysate