Mouse Subcellular Fraction Specific Blot
The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.
The premade blots are prepared with the appropriate GenLysate™ and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient™ Western Transfer Buffer [20X].
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate™.
Blot lane order:
- Protein marker
- Mouse Whole Liver
- Mouse Nuclei Lysate
- Mouse Mitochondria Lysate
- Mouse Golgi Complex Lysate
- Mouse Smooth Endoplasmic Reticulum Lysate
- Mouse Rough Endoplasmic Reticulum Lysate
- Ready-to-screen western tissue blot