Well-Coated™ Amine Binding plates are designed to specifically bind primary amines of peptides, proteins and other molecules. The Well-Coated™ Amine Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for binding assays.
Well-Coated™ Amine Binding plates are maleic anhydride activated plates that react with primary amines to form amide bonds that are stable at pH≥7. Acidic conditions will hydrolyze the bonds releasing the peptide/ligand, therefore binding of peptide/ligand to plates should be performed at pH8-9 and the binding assays or ELISA should be performed at pH≥7.
The wells are coated to a 200µl depth. The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively. The clear plates are 8-well strip plates.
Features
- Rapid and spontaneous binding of primary amines
- Stable plates, for immobilizing molecules by covalent attachment to the plate for ELISA and other assays
Applications
- For the binding of peptides, proteins and other ligands through primary amines
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