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femtoLUCENT™ PLUS-AP (3 Citations)


The femtoLUCENT™ PLUS-AP kit is based on alkaline phosphatase (AP) chemiluminescence reaction. The chemiluminescence light emission can be recorded by a short exposure to blue-light sensitive autoradiography films. The femtoLUCENT™ PLUS-AP kit allows for detection of femtogram quantity of antigens with no background. The kit reagents are sufficient for 25 mini blots (8.5 x 7.5 cm size).  

 

In addition, femtoLUCENT™ PLUS-AP is also supplied in a kit format, containing our non-animal protein blocking agent (NAP BLOCKER™) and  wash buffer (femto-TBST™).  The femtoLUCENT™ PLUS-AP kits allow detection of low femtogram levels (10-15) of antigens with low noise (signal/background) ratio.  The kit reagents are sufficient for 25 mini blots or 1,500cm2 of PVDF or nitrocellulose membrane.  The trial size (Cat. # 786-10APT) is suitable for 5 mini blots or 300cm2.

 

For a complete chemiluminescence immunodetection system, we offer optional alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat. 

 


Features

  • Intense light emission with low background and high signal
  • Supplied with a novel blocking agent to allow a rapid blocking step that produces a clear background
  • Economical: greater value compared to similar products

 


Applications

  • Quick and sensitive non-isotopic detection of proteins on transfer membranes (suitable for nitrocellulose & PVDF membranes)
  • Immunodetection of proteins and antigens for Western blots and dot blot applications
  • Low femtogram detection (10-15) that allows for detection of >10fg protein on a dot blot and >1pg on a Western blot

 

 

 

Protocol
786-10AP
786-10APT
Material Safety Data Sheet
786-10AP
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786-10APT
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish

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