TCEP-HCl; Tris (2-carboxyethyl) phosphine-HCl

TCEP is a water-soluble, odorless, non-toxic and stable protein reductant. This potent reducing agent is a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol) use for most applications. The reduction potency of TCEP is twice as high as that of DTT, and TCEP is effective in reducing proteins over a wider range of pH conditions, including lower acidic pH ranges (pH 2-11), compared to other reductants.

Since TCEP is a charged molecule, it cannot penetrate hydrophobic environments. Therefore, the use of a denaturant, acidic condition (pH 2.8-5) or basic condition (pH 9-11), is recommended for effective reduction.

TCEP reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions.

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Features

• Chemical Name: tris(2-carboxyethyl)phosphine, HCl
• Synonym: TCEP, Hydrochloride
• CAS Number: 51805-45-9
• Chemical Formula: C₉H₁₅O₆P•HCl
• Molecular Weight: 286.65
• Form: White crystalline solid
• Solubility: H₂O (5mg/ml)

Applications

• Reduction of protein and peptide solutions