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N-Acetyl-D-Glucosamine Agarose

Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and other technologies. Some of the possible or ongoing applications of lectins are use as pesticide, fungicide, drug for cancer and antiviral drug. Lectins are used in affinity chromatography for purification of glycoproteins, carbohydrates and glycolipids. Lectins are also useful as markers for blood typing, therefore purification of lectins is the important.

 

N-Acetyl-D- Glucosamine Agarose is used in purification of N-acetyl-D- glucosamine specific lectins and carbohydrate binding proteins/enzymes. Examples of N-acetyl-D- glucosamine specific lectins are wheat germ agglutinin, elderberry lectin, Maackia amurensis lectin, etc.

 

N-Acetyl-D-Galactosamine agarose and Immobilized D-galactose are also available.

 

 


Features

  • Ligand: N-acetyl-D-glucosamine
  • Matrix bead structure: 6 % cross-linked agarose
  • Matrix bead size: 45-160 mm
  • Matrix activation: Epoxy
  • Matrix binding to ligand: via –OH groups
  • Spacer arm: 12 atoms
  • Binding capacity: ≥ 25 mg wheat germ lectin/ml of resin

 



Applications

  • N-Acetyl-D-Glucosamine Agarose is used for purification of wheat germ (Triticum vulgaris) lectin, other N-acetyl-glucosamine specific lectins such as lectin from Dolichos biflorus and carbohydrate binding proteins/enzymes.

 

 

Protocol
786-270
Material Safety Data Sheet
786-270
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