Immobilized Protein G (2 Citations)
Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of Streptococcal group G so that it does not bind to albumin. Protein G is coupled to 4% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for Ig and minimal protein G leaching. Our immobilized protein G has a high binding capacity (>20mg sheep IgG/ml resin).
Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
The Immobilized Protein G 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. Each 0.2ml column enables purification of 0.1-1mg IgG from 25-500µl of serum or other sample.
The Immobilized Protein G 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. Each 1ml column enables purification of 1-13mg IgG from 0.5-2ml of serum or other sample.
Also available in 96-well spin plate formats for processing up to 96 samples.
Specific binding and elution buffers are also available.
- High binding capacity: 38mg human IgG/ml resin; >20mg sheep IgG/ml resin
- Ligand: Recombinant Streptococcal Protein G lacking the albumin-binding domain produced in E. coli
- Bead size: 50-165μm
- Bead Structure: 4% highly cross-linked agarose
- Maximum flow rate 1,000cm/hr
- Maximum pressure 300kPa
- Suitable for immunoaffinity chromatography and immunoprecipitation.
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