PEROXsay™ Peroxide Assays (4 Citations)
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the PEROXsay™ assay allows the ferric (Fe3+) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
PEROXsay™ is suitable for quantifying the level of protein damaging peroxides in biological solutions, measurement of lipid peroxidation of low density lipoproteins and liposomes and monitoring protein glycation.
The PEROXsay™ assay is designed for microtiter plates, but can be scaled up for use with 1ml cuvettes.
PEROXsay™-LIPID permits the quantification of peroxides in the presence of lipids without the need for extraction steps.
- Colorimetric Assay
- Linear Range: 0-50µM
- Highly Reproducible
- Enzyme Independent
- Rapid, Accurate & Inexpensive
- Assay peroxide concentrations in biological solutions
- Measure lipid peroxidation
- Monitor protein glycation
- Alaminis-castillo, M.A. and Ho-plagaro, A . (2019) Int. J. Clin. Pract. Increased PON lactonase activity in morbidly obese patients is associated with impaired lipid profile.doi.org/10.1111/ijcp.13315
- Devillard, E. et al (2004) J Bacteriol 186:136
- Rincon, M. et al (2004) J Bacteriol 186:2576
- Shen, J. et al (2004) Cancer Res. 64:9018