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PEROXsay™ Peroxide Assays (4 Citations)

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the PEROXsay™ assay allows the ferric (Fe3+) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
 
PEROXsay™ is suitable for quantifying the level of protein damaging peroxides in biological solutions, measurement of lipid peroxidation of low density lipoproteins and liposomes and monitoring protein glycation.
 
The PEROXsay™ assay is designed for microtiter plates, but can be scaled up for use with 1ml cuvettes.
 
PEROXsay™-LIPID permits the quantification of peroxides in the presence of lipids without the need for extraction steps.

 

 


Features

  • Colorimetric Assay
  • Linear Range: 0-50µM
  • Highly Reproducible
  • Enzyme Independent
  • Rapid, Accurate & Inexpensive

 


Applications

  • Assay peroxide concentrations in biological solutions
  • Measure lipid peroxidation
  • Monitor protein glycation

 

 

Protocol
786-440
786-441
Material Safety Data Sheet
786-440
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Danish
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English_UK
French
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Portuguese
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786-441
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish

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PEROXsay™ Peroxide Assays
PEROXsay™ Peroxide Assays
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions. Peroxides reac..
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