1-Fluoro-2,4-dinitrobenzene [DNFB], commonly known as Sanger’s reagent is a chemical amino acid modifier that reacts with N-terminal amino acid of polypeptides. In Sanger reaction DNFB is used to form dinitrophenyl derivatives for end group determination of proteins and to derivatize primary amines.
- Molecular Formula:C6H3FN2O4
- Molecular Weight: 186.1
- CAS Number: 70-34-8
- Melting point: 26°C
- Density: 1.48/ml
- Molarity: 7.95 M
- Solubility: Soluble in chloroform at 100 mg/ml. Also soluble in benzene, ether and propylene glycol.
- Storage/Stability: The half life at 15°C in Tris buffer is 56.7 hours at pH8.03 and 6.5 hours at pH9.53
- Used in Sanger reaction to form dinitrophenyl derivatives for end group determination of proteins and to derivatize primary amines.
- Depending upon its property of reaction with N-terminal amino acid of polypeptides DNFB can also be used to inhibit activity of some enzymes.