G-Trap™ Protein G

G-Trap™ Protein G are prepacked ready to use columns for affinity purification of Immunoglobulins G. The column is packed with resin, which is covalently coupled to recombinant Protein G.

Recombinant Protein G is coupled covalently by proprietary coupling method to highly crosslinked 6% agarose. The coupling chemistry used shows high coupling efficiency and low Protein G leaching and thus high binding efficiency. The recombinant Protein G, produced in E. coli, has been designed to genetically remove albumin and cell surface binding regions of the protein.  

 G-Trap™ rProtein A FF, 1 ml and G-Trap™ rProtein A FF, 5 ml are also available for affinity purification of Immunoglobulins.

The properties of the column and resin are listed below

G-Trap™ Protein G resin

• Mean particle size: 90 µM
• Bead Structure: Highly cross-linked 6% agarose
• Ligand: Recombinant Streptococcal Protein G lacking the albumin binding domain produced in E. coli
• Binding capacity: 38mg human IgG/ml resin ; >20mg sheep IgG/ml resin
• Maximum flow rate: 1000 cm/hr
• Chemical Stability:Stable in all commonly used buffers
• pH stability: Long term: pH 3-9, short term pH 2-9
• Storage: 2°C to 8°C in 20% ethanol

G-Trap™ Protein G, 1 ml columns

• Column Volume: 1 ml
• Columns Dimension: 0.7 x 2.5 cm
• Column Harware Pressure Limit: 0.5 MPa
• Column Hardware: Polypropylene

G-Trap™ Protein G, 5 ml columns

• Column Volume: 5 ml
• Columns Dimension: 1.6 x 2.5 cm
• Column Harware Pressure Limit: 0.5 MPa
• Column Hardware: Polypropylene

Application(s): 

• G-Trap™ Protein G are prepacked ready to use columns for affinity purification of Immunoglobulins G