For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their primary amines.
The amine reactive HOOK™
Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by reductive amination, using sodium cyanoborohydride
to form stable amine linkages between the agarose and the ligand.
- Binding capacity: 20mg protein/ml resin
- 6% cross-linked agarose
Coupling of proteins and peptides to agarose beads
Suitable for antibody purification.
Discovery of new interacting proteins.