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Labeling & Conjugation

G-Biosciences offer a vast array of products for protein labeling and conjugations. Both fluorescent and biotin labeling kits are available as well as products for agarose conjugation and accessories. 

Fluorescent dyes have become a common tool in laboratories for the visualization and studying of proteins, whether they are attached directly to the protein of interest or to antibodies specific for the protein of interest. 

G-Biosciences researchers have developed two kits for the rapid and efficient labeling of proteins, including antibodies, with a fluorescent green (FITC) or red ((5/6) TAMRA-SE) dye.  Each kit is supplied with five of our single-use OneQuant™ fluorescent reagents that protect the dyes from exposure to light and minimize hands-on time.  The following evaluates labeling of two different proteins with fluorescent dyes.

 

HOOK-Biotin kits come with all the necessary reagents and equipment for optimization of reaction conditions, efficient labeling, removal of unbound biotin and quantification of biotin labeling.  In addition to highly efficient labeling, the HOOK-Biotin kits offer the advantage of being supplied with micro dialysis units and a specific Optimizer Buffer.  These greatly simplify the labeling process and ensure high levels of biotin labeling.

 

Download the Protein Labeling & Conjugation Handbook

Gene/Protein:
Avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates. Avidin has a molecular weight of 67kDa and contains four identical 128 amino acid subunits that each have a single biotin binding domain. Avidin is a basic protein with an isoelectric pH of 10-10.5 and is rea..
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A biotin estimation dye reagent...
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An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers a complete kit for the coupling of peptides and proteins to agarose through thei..
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HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein.   The method of biotin incorporation estimation ..
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HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. &..
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Gene/Protein:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides. (5/6) TAMRA absorbs green visible light at 546nm and emits an o..
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Gene/Protein:
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 52..
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HOOK™ Biotin-BMMCC reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl res..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the sel..
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The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research. Several factors must be considered when coupling a biotin reagent to a protein to ensure a successful reaction. The primary consideration is the sel..
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HOOK™ Biotin-PDA reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of the amino acid cysteine is the most common source of free sulfhydryl groups. If free sulfhydryl resid..
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