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HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine) (8 Citations)


(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.

(5/6) TAMRA absorbs green visible light at 546nm and emits an orange-red visible light at a maximum emission of 575nm.

The NHS ester group provides the simplest and most commonly used group for labeling proteins. The succinimidyl ester group reacts with primary amines in lysine side chains and N-terminal amines forming a stable, covalent amide bond.

This kit utilizes SpinOUT™ desalting columns, for the rapid removal of unconjugated dye.
 
The (5/6) TAMRA-SE labeling reagent is also available in our single use OneQuant™ format.
 
This kit is an improvement on Cat. # 786-058 as it offers greater free dye removal and complete coupling in less time.

 


Features

  • Dye preweighed and supplied in single use OneQuant™ vials (Also available separately).
  • Suitable for most proteins.
  • Utilizes  SpinOUT™ desalting columns, for the rapid removal of unconjugated dye

 


Applications

  • Labeling of proteins, peptides and nucleic acids with a red fluorescent dye.
  • Suitable for antibody labeling.

 

 

Protocol
786-079
786-142
Material Safety Data Sheet
786-079
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-142
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Antibody Handbook
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Protein Labeling & Conjugation

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