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HOOK™ Dye Labeling Kit (FITC) (4 Citations)


FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light. This kit utilizes SpinOUT™ desalting columns, for the rapid removal of unconjugated dye.

 
The FITC labeling reagent is also available in our single use OneQuant™ format.
 

This kit is an improvement on Cat. # 786-059 as it offers greater free dye removal and complete coupling in less time.

 


Features

  • Synonym:  Fluorescein-5-Isothiocyanate
  • CAS Number: 3326-32-7
  • Linear Formula: C21H11NO5S
  • Molecular Weight: 389.4
  • Dye preweighed and supplied in single use OneQuant™ vials (Also available separately).
  • Suitable for most proteins.
  • Utilizes  SpinOUT™ desalting columns, for the rapid removal of unconjugated dye

 


Applications

  • Labeling of a green fluorescent dye to proteins and peptides.
  • Suitable for antibody labeling.

 

 

Protocol
786-080
786-141
Material Safety Data Sheet
786-080
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-141
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Antibody Handbook
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Protein Labeling & Conjugation

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