Streptavidin is a non-glycosylated 52.8 kD tetrameric protein, which binds to biotin and is obtained from the bacteria Streptoimyces avidinii. When compared to Avidin, Streptavidin exhibits less non-specific binding than avidin. The dissociation constant (KD) of Streotavidin-biotin complex is around 10-14 M, which is one of the strongest non-covalent bonds.
G-Biosciences HOOK™ Maleimide Activated Streptavidin is offered to enable its conjugation with fluorescent dyes or ELISA enzymes such as horseradish peroxidase (HRP) containing sulfhydryl (-SH) groups. Streptavidin conjugated with dyes or enzymes is frequently used in DNA hybridization techniques, immunohistochemistry, ELISA and flow cytometry. Biotin conjugated probes or primary antibodies are used to localize antigen and streptavidin conjugated to fluorescent probe or enzyme binds to biotin and detects the antigen. Besides this streptavidin can also be conjugated to antibodies to localize antigen and biotin conjugated to the detection molecules.
Streptavidin is maleimide activated using Sulfo-SMCC, a heterobifunctional crosslinker which adds a free maleimide group, which reacts with sulfhydryl containing molecules.
- Sulfo SMCC activated Sterptavidin
- Sulfhydryl Reactive: Conjugates with molecules containing reduced thiol (-SH) groups.
- Available as ready to use 1 mg lyophilized vial.
- HOOK™ Maleimide Activated Streptavidin conjugates with dyes or enzymes is frequently used in DNA hybridization techniques, immunohistochemistry, ELISA and flow cytometry.