Taq DNA Polymerase (5 Citations)

Catalog
Description
Size
Price(USD)
Qty
Catalog
786-447
786-447
Description
Taq DNA polymerase
Taq DNA polymerase
Size
1000U
1000U
$87.00
$87.00
Catalog
786-448
786-448
Description
Taq DNA polymerase
Taq DNA polymerase
Size
5 X 1000U
5 X 1000U
$335.00
$335.00
Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile, Thermus aquaticus. The molecular weight of the recombinant protein is 94kD.. The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. The error rate of this Taq polymerase is ~2.2x10-5 nucleotide-1 cycle-1. Taq DNA polymerase catalyzes the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product
SOURCE
A recombinant protein expressed in E. coli that carries the pol gene for Thermus aquaticus.
SOURCE
A recombinant protein expressed in E. coli that carries the pol gene for Thermus aquaticus.
STORAGE BUFFER
20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl2, 1mMDTT, 0.1% Nonidet® P-40, 0.1% Tween® 20, 0.2mg/ml BSA, 50% (v/v) glycerol.
10X PCR BUFFER
100mM Tris-HCL pH8.8, 500mM KCl, 16mM MgCl2, 1% TritonX-100
UNIT DEFINITION
One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate.
10X PCR BUFFER
100mM Tris-HCL pH8.8, 500mM KCl, 16mM MgCl2, 1% TritonX-100
UNIT DEFINITION
One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate.
Our Taq DNA Polymerase is offered in four convenient sizes and supplied with 10X PCR buffer (Mg2+ plus).
Taq Polymerase 2X Mastermix is a ready-to-use mixture of our high quality Taq DNA polymerase, deoxynucleotides and reaction buffer at a 2X concentration.
Features
- 5’→3’ polymerase activity
- No detectable 3’→5’ proofreading exonuclease activity
- Low 5’→3’ exonuclease activity
-
Error rate ~2.2x10-5 nucleotide-1 cycle-1
-
Available as a 2X Mastermix
Protocol | |
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Material Safety Data Sheet | |
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786-448 |
Technical Literature | |
Molecular Biology Handbook | A guide to our products for DNA and RNA. |
- "Kiran, R. et al (2022) Development of multiplex PCR assay for detection of Alernaria brassicae, A. brassiciola and Xanthomonas campestris pv. campestris in crucifers. ARCH MICROBIOL. https://doi.org/10.1007/s00203-022-02846-5"
- Jangra, Sumit et al (2021) Promising versions of a commercial pearl millet hybrid for terminal drought tolerance identified through MAS. J GENET. https://doi.org/10.1007/s12041-021-01337-8
- Kumar, T.P.J. et al (2021) Development of near isogenic lines for grain softness through marker assisted backcross breeding in wheat. J PLANT BIOCHEM BIOTECHNOL. https://doi.org/10.1007/s13562-021-00712-x
- Rohini. M. R. et al (2020) Morphological characterization and analysis of genetic diversity and population structure in Citrus × jambhiri Lush. using SSR markers. Genet Resour Crop Evol.doi.org/10.1007/s10722-020-00909-4
- GG JAT (2019) qPCR analysis of Ty-2 and Ty-3 gene pyramided lines of tomato for resistance to tomato leaf curl New Delhi virus (ToLCNDV). INDIAN J AGR SCI. 2019
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