menu
Your Cart

Taq Polymerase

Catalog Description Size Price(USD) Qty
786-447
Taq DNA polymerase 1000U
$102.00
786-448
Taq DNA polymerase 5 X 1000U
$385.00

Taq Polymerase

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile, Thermus aquaticus.  The molecular weight of the recombinant protein is 94kD..  The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. The error rate of this Taq polymerase is ~2.2x10-5 nucleotide-1 cycle-1. Taq DNA polymerase catalyzes the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

SOURCE
A recombinant protein expressed in E. coli that carries the pol gene for Thermus aquaticus.
 
STORAGE BUFFER
20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl2, 1mMDTT, 0.1% Nonidet® P-40, 0.1% Tween® 20, 0.2mg/ml BSA, 50% (v/v) glycerol.

10X PCR BUFFER
100mM Tris-HCL pH8.8, 500mM KCl, 16mM MgCl2, 1% TritonX-100 

UNIT DEFINITION
One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate.
 
 
Our Taq DNA Polymerase is offered in four convenient sizes and supplied with 10X PCR buffer (Mg2+ plus).
 
Taq Polymerase 2X Mastermix is a ready-to-use mixture of our high quality Taq DNA polymerase, deoxynucleotides and reaction buffer at a 2X concentration.
 

 


Features

  • 5’→3’ polymerase activity
  • No detectable 3’→5’ proofreading exonuclease activity
  • Low 5’→3’ exonuclease activity
  • Error rate ~2.2x10-5 nucleotide-1 cycle-1
  • Available as a 2X Mastermix

 

 

Protocol
786-447
786-448
Material Safety Data Sheet
786-447
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-448
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Molecular Biology Handbook  A guide to our products for DNA and RNA.
Write a review
Note: HTML is not translated!
Bad Good
Captcha

Download our handbooks

Custom Services

Connect With Us

Stay up to date with G-Biosciences by signing up for our newsletter.