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Protease Assay Kit (4 Citations)

The Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample, using a dye-labeled protein substrate.

The proteases present in the sample of interest will digest the protein substrate and release dye labeled peptides. The absorbance of the dye-labeled peptide is measured at 570nm for determination of protease activity.

Chemically stabilized Trypsin (MSG-Trypsin™) is supplied with the kit as a general protease standard; however, other specific protease standards can also be used. MSG-Trypsin™ is an ultra-pure trypsin from bovine pancreas, modified by methylation followed by TPCK treatment and is extremely resistant to autolysis.

The kit components are sufficient for 50 assays in a microtiter plate format or 0.5 milliliter assay tubes.

 


Applications

  • Determination of protease activity in biological samples, with nanogram detection levels

 

 

Protocol
786-028
Material Safety Data Sheet
786-028
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Technical Literature
Bioassays Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protein Assay Handbook & Selection Guide  An introduction to protein assays.

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