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BLOT-FastStain™

Catalog Description Size Price(USD) Qty
786-34
BLOT-FastStain™ 25 Blots
$234.00

BLOT-FastStain™

A unique stain for reversibly staining protein on nitrocellulose and PVDF transfer membranes.
 
BLOT-FastStain™ only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility. The staining procedure takes 10 minutes and has a sensitivity of 2ng BSA, higher than silver stains. Destain the membrane by simply rinsing in warm water for 10 minutes.
 
Staining and destaining does not affect the biological properties of the proteins. After destaining, protein bands can be probed with Western blot protocols and other analyses including sequencing work.
 
BLOT-FastStain™ is fully compatable with the LiCor Odyssey detection system and has succesfully been used as a loading control in this Odyssey system.

 


Features

  • Reversible stain for protein membranes
  • Compatible with PVDF and nitrocellulose membranes
  • Detect >2ng protein

 

Applications

  • Staining of membranes for visualization of proteins onto the membrane after Western transfer and dot-blot applications

 

 

Protocol
786-34
Material Safety Data Sheet
786-34
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Technical Literature
Membrane Stains for IR Imaging Infrared (IR) Imaging of Western blots is a fast growing application with several companies offering imaging system and near infrared fluorophores.  IR imaging offers many benefits over chemiluminescence and as a result popularity is growing.   Despite the advancement in these detection system, the quality of the blot is still crucial and proper controls should be maintained.  One key control is loading controls.  Total protein stains are become widely accepted as loading controls over measuring housekeeping genes.  The main reasons for this are: Take into account total protein profile not one single protein Fast, takes a few minutes as opposed to hours for antibody development Affordable as no expensive antibodies required Reversible, total protein stains can be completely removed   The big question is "Are there IR Imaging System Compatible Stains?"   Download the free application note to review independent data on the use of G-Biosciences reversible membrane stains!
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
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