menu
Your Cart

BCA Protein Assay (54 Citations)

BCA Protein Assay
BCA Protein Assay








The BCA Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid (BCA) chelate with each Cu+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562 nm that is linear for increasing protein concentrations between the range of 0.02-2mg/ml. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations. The Bicinchoninic Acid (BCA) Protein Assay is suitable for quantifying protein solutions in 1ml assays or in micro-wells.

Features

  • Sensitive colorimetric assay
  • Linear range of 20-2,000µg/ml
  • Compatible with wide range of ionic and non-ionic detergents
  • 1ml cuvette or micro well compatible

Applications

  • For the estimation of protein concentration
The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.
 
For dilute protein samples (0.5-20µg/ml), we recommend the Micro Bicinchoninic Acid (BCA) Protein Assay
 
For samples with reducing agents, we recommend the Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay.
Protocol
786-570
786-571
786-890
786-891
786-844
786-845
786-846
786-847
786-848
Material Safety Data Sheet
786-570
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-571
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-890
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-891
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-844
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-845
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-846
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-847
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-848
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
A Non-Animal Alternative to BSA Protein Standards
Bioassays Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.

Write a review

Note: HTML is not translated!
Bad Good
Captcha

CONNECT WITH US