ProteaseArrest™ Protease Inhibitor Cocktail (209 Citations)

A broad spectrum protease inhibitor cocktail solution that is provided as a 100X concentrated, ready-to-use solution. The cocktail contains a mix of reversible and irreversible inhibitors of serine, cysteine, calpain and metallo- proteases, including Aprotinin, PMSF, Leupeptin, Bestatin and AEBSF.  An optional EDTA solution is provided for enhanced metalloprotease inhibition, and is provided separate as it would inhibit the activity of proteins that require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity and will inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC).

The ProteaseArrest™ 100X solution format is suitable for small, analytical sample applications, as >95% inhibition is achieved by adding 10µl ProteaseArrest™ per ml sample. For samples with higher than normal protease levels, the volume of ProteaseArrest™ added can be increased for greater inhibition levels. Protease levels can easily be assayed with our Protease Assays. Due to the optimized concentration of the various inhibitors, ProteaseArrest™ shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during preparation of samples and protein purification from animal tissues, plants, yeast and bacteria.

ProteaseArrest™ is also available as single use aliquots that are suitable for >95% protease inhibition in 10ml solutions. These OneQuant™ ProteaseArrest™ are provided for additional protease inhibitor cocktail convenience.   ProteaseArrest™ is also offered as a dry format, as ProteCEASE™, for those who prefer reconstitution prior to use.

ProteaseArrest™ Outperforms Tablet Cocktails

The ProteaseArrest™ format allows delivery of optimized concentrations of protease inhibitor, for example 2X or higher concentrations can be added for tissues with higher than normal protease concentrations; a feature not possible with tablet format protease inhibitor cocktails. In our study, a 1X concentration of ProteaseArrest™ inhibits over 95% of protease activities (e.g. Mouse Pancreas Extract: 0.5 milligrams per milliliter of protein). The ProteaseArrest™ protease inhibitor cocktails demonstrate greater inhibition levels compared to similar protease inhibitor cocktails, including tablet formats (see figure). In independent studies, researchers have found that ProteaseArrest™ outperforms several leading manufacturer's protease inhibitor cocktails, including tablet formats, in the purification of plant proteins (1). 

G-Biosciences Protease-Phosphatase Arrest provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Our Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with 5  phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

Features

• Broad spectrum protease inhibitor cocktail with high inhibition levels: 1X concentration inhibits >95% of protease activities
• 100X concentrated, ready-to-use solution, just pipette what you need. No wastage.
• Versatile as a separate vial of EDTA, a metalloprotease, is provided to protect proteins that require metal ions or if IMAC is a downstream application.
• No proprietary ingredients, fully disclosed formulation of six broad range protease inhibitors, including AEBSF, Aprotinin, Bestatin, EDTA, Leupeptin and PMSF
• Multiple package sizes, including single-use packaging
• Compatible in nearly all lysis buffers.

Applications

• Complete inhibition of protease activities during protein preparations
• Suitable for yeast, plant, bacteria, fungal & animal tissue lysates
• Protection of proteins from proteolysis during preparation of samples, protein purification, electrophoresis, storage, assays & other applications

ProteaseArrest Family