Mammalian ProteaseArrest
Inhibits serine, cysteine and mammalian specific proteases, including
aminopeptidases, aspartic, cysteine and trypsin-like proteases.
Mammalian ProteaseArrest contains agents to
inhibit serine, cysteine and
mammalian specific proteases, including aminopeptidases, aspartic, cysteine and
trypsin-like proteases. Mammalian
ProteaseArrest contains both irreversible and reversible protease inhibitors
and an optional EDTA solution is provided for inhibition of metalloproteases.
Due to the optimized
concentration of the various inhibitors, Mammalian
ProteaseArrest shows excellent and specific inhibition of mammalian protease
activities and is therefore suitable for the protection of proteins during
preparation of samples and protein purification from animal tissues and cells.
Mammalian ProteaseArrest is supplied with an
optional EDTA solution for the inhibition of metalloproteases and to preserve
the activity of proteins that require divalent cations (Ca2+, Mg2+,
or Mn2+) for their biological activity. Furthermore, EDTA interferes with IMAC
(immobilized metal affinity chromatography), a common purification technique,
so the optional EDTA can be omitted if IMAC is to be used.
Ready-to-use solution: Simply
add an appropriate volume of Mammalian
ProteaseArrest in protein extract or extraction buffer to inhibit protease
activity.
Supplied
as a 100X solution, recommend 1X for final sample volume.
FEATURES
A mammalian specific protease inhibitor cocktail.
Ready-to-Use
Supplied as 100X solution.
APPLICATIONS
- Inhibition
of protease activity in mammalian protein preparations.
- Protection of proteins from proteolysis in
such applications as electrophoresis, purification, storage, assays, and
other applications.
