G-Sep™ Agarose CL-4B

G-Sep™ Agarose CL-4B resin is a cross-linked versions of our G-Sep™ Agarose. Cross-linking of the agarose results in chemically and physically more stable agarose beads that offer the same selectivity, but with better flow characteristics. Cross-linked agarose beads are resistant to organic solvent.

G-Sep™ Agarose CL-4B is a gel filtration matrix formed from agarose beads that is available with 4% cross-linked agarose content, designated G-Sep™ Agarose CL-4B.

The resin is a proven gel filtration base matrix and is routinely modified by researchers to couple affinity ligands. The resin is not pre-activated.

Our G-Sep™ Agarose CL is also available with 6% cross-linked agarose content, designated G-Sep™ Agarose CL-6B.

Features

• Matrix: 4% cross-linked agarose
• Bead form: Spherical, diameter 50-160µm
• pH stability: 3-13
• pH stability cleaning-in-place (CIP): 2-14
• Maximum pressure (MPa): 0.012
• Maximum flow velocity: 26cm/h
• Fractionation [Mr] globular proteins: 6x10⁴ - 2x10⁷
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• Chemical stability: 6M urea, 8M guanidine hydrochloride, ethanol, DMF, THF, acetone, DMS, chloroform, dichloromethane, dichloroethane, pyridine, triethyl phosphate and acetonitrile.
• Sterilization: Autoclavable, 121°C, pH 7, for 20 min
• Storage condition: 4-30ºC, 20% ethanol

Applications

• Gel filtration media
• Base matrix for coupling affinity ligands