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Concanavalin A (Con A) Agarose




Concanavalin A (Con A) Agarose consists of Con A coupled to agarose by the cyanogen bromide method.  Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean).

 

Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues.  Con A agarose has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles.

 

As stated above, Con A is a metalloprotein and to maintain its binding characteristics the presence of both Mn2+ and Ca2+ is essential. Each subunit of Con A utilizes one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.
 

 

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Features

  • Binds α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues
  • Purification/ enrichment of of glycoproteins, polysaccharides and glycolipids
  • Ligand Density: 10-16mg Con A/ml resin
  • Capacity: 20-50mg thyroglobulin/ml resin
  • Bead structure: 6% agarose

 


Applications

  • Purification/ enrichment of of glycoproteins, polysaccharides and glycolipids

 

 

Protocol
786-208
786-216
786-217
786-218
Material Safety Data Sheet
786-208
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786-216
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-217
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-218
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Protein Purification Handbook

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