Well-Coated™ Biotin plates are designed to specifically bind avidin, streptavidin or Neutravidin™ conjugated molecules, including enzyme conjugates.
Biotin exhibits an extraordinary binding affinity for avidin (Ka=1015M-1) and streptavidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by autoclaving. Streptavidin and Neutravidin™ in many respects are similar to avidin except that they have no carbohydrate and their solubility in aqueous buffer is much lower than avidin. Neutravidin™ also lacks the RYD sequence eliminating interaction with RGD domain of adhesion receptors. The binding of strepatavidin and Neutravidin™ is similar to that of avidin, but with less non-specific binding.
The wells are coated to a 200µl depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer. The plates have the capacity to bind 30-50ng streptavidin per well.
The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.
- Binds avidin, streptavidin and Neutravidin™ conjugated molecules
- Reduced non-specific binding as plates are pre-blocked with Superior™ Blocking Buffer