The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation. Many common buffers routinely used in laboratories have an i..
Avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates. Avidin has a molecular weight of 67kDa and contains four identical 128 amino acid subunits that each have a single biotin binding domain. Avidin is a basic protein with an isoelectric pH of 10-10.5 and is rea..
Hydroxylamine•HCl is a reducing agent that is routinely used for the deacetylation of SATA to form free sulfhydryls (Figure 1), for cleavage of protein cross-linkers that contain carbonyl groups (i.e. EGS) and for mutagenesis of plasmid DNA.
L-Cysteine hydrochloride salt is routinely used with Ellman's Reagent assays as a sulfhydryl standard. In addition, it is also used as a supplement for protein refolding experiments.
CAS #: 7048-04-6
Molecular weight: ..
Purified and commercial antibodies are routinely stored in buffers containing bovine serum albumin (BSA) and gelatin that act as stabilizers during long term storage. In routine applications, such as ELISA, Western blotting and other immunodetection techniques, these proteins generally do not i..
Sodium metaperiodate, or sodium m-periodate, is a mild oxidant that is routinely used for the conversion of cis-glycol groups in carbohydrates to reactive aldehdye groups (Figure 1). The reactive aldehyde groups are used in chemical conjugation procedures or detection of carbohydrates. F..
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The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, prot..
Tube-O-Reactor™ is a system that allows all the key steps of cross linking, coupling and modification of proteins and/or nucleic acids to be performed in a single tube. This minimizes the risk of sample loss, of experimental time and of hands-on phases.
Most of the above reactions..
Well-Coated™ Biotin plates are designed to specifically bind avidin, streptavidin or Neutravidin™ conjugated molecules, including enzyme conjugates.
Biotin exhibits an extraordinary binding affinity for avidin (Ka=1015M-1) and streptavidin (Ka=1015 M-1). Biotin a..