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HOOK™ BiotinQuant™

Catalog Description Size Price(USD) Qty
BKC-01
HOOK™ BiotinQuant™ Kit 20 Assays
$172.00

HOOK™ BiotinQuant™

HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein.

 

The method of biotin incorporation estimation is based on the binding of avidin with HABA dye, which produces a color that can be read at 500nm. The HABA-avidin complex can be displaced with free biotin or biotin conjugated with other molecules (proteins). Measuring the change in optical density of HABA-avidin complex with biotinylated proteins allows for accurate estimation of the molar ration of biotin conjugated to the protein/ antibody.

 

HOOK™ BiotinQuant™ is supplied with premixed, OneQuant™ HABA/Avidin aliquots to eliminate difficulties in weighing small amounts of reagents and ensuring reproducible estimations.

 

Click links for a selection of biotin labeling reagents and complete kits

 


Features

  • Accurate estimation of biotin incorporation
  • Supplied with OneQuant™ HABA/Avidin to eliminate waste for weighing small quantities

 


Applications

  •  Estimation of biotin labeling of antibodies and other proteins

 

 

Protocol
BKC-01
Material Safety Data Sheet
BKC-01
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Technical Literature
Antibody Handbook
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Protein Labeling & Conjugation
Protein Purification Handbook
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