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Western ReProbe™ PLUS

Catalog Description Size Price(USD) Qty
786-307
Western ReProbe PLUS 500ml
$126.00
786-308
Western ReProbe PLUS 1L
$234.00
786-309
Western ReProbe PLUS 1 Gallon
$437.00

Western ReProbe™ PLUS

Based on our popular Western ReProbe™, the modified formulation allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity.  The membrane bound protein is retained on the membrane and the matching antibodies are washed away.  Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

 

Each 100ml of Western ReProbe™ PLUS is sufficient for 25-30 standard size (7.5cm x 8.5cm) western blots.

 

Western ReProbe™ PLUS is not recommended for stripping color producing Western blots that use substrates such as TMB, chloronapthol and DAB. 

 


Features

  • Remove high affinity antibodies
  • Simply incubate the blot in Western ReProbe™ PLUS at room temp & wash
  • No boiling, denaturants or SDS required
  • Repeated analysis of Western blots with multiple probes
  • Re-analysis & correction of unsatisfactory Western blots
  • Conservation of hard-to-obtain test samples & reagents
  • Time saving in not rerunning gels & transfer processes

 


Applications

  • Screen same blot with different antibodies
  • Reprobe for housekeeping proteins
  • Compare phosphorylated & total protein on the same blot

 

 

Protocol
786-307
786-308
786-309
Material Safety Data Sheet
786-307
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786-308
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-309
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Multiple Probing of Western Blots
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
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