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ECH-Agarose


ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting in 15-atom hydrophilic spacer arm. 

 

Amine groups of the ligands bind to the ECH-Agarose by carbodiimide coupling method. In this coupling method the free carboxyl group of the resin reacts with amine group of the ligand to form a stable peptide bond in the presence of water soluble carbodiimide such as EDC (N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride) at low pH (4-5). 

 

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Features:

  • Resin Active group: -COOH groups
  • Group to be coupled: -NH2 groups  
  • Resin Active group density: 50 µM carboxyl/ ml drained resin.
  • 45-165µm particle size range
  • Spherical, cross-linked agarose
  • Resin spacer arm: 15 atom hydrophilic spacer arm.
  • Coupling conditions: at 4°C- 25°C, pH: 4.5 -6 for 1.5 -24 hrs, Coupling could be done in organic solvents.
  • No blocking reaction is required after the coupling reaction, saves time
  • Storage: 2- 8°C

 

Application(s):

  • ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography
  • ECH-Agarose provides 15 atom hydrophilic spacer arm that enables coupling of small ligands containing amine groups
Protocol
786-1223
786-1224
Material Safety Data Sheet
786-1223
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786-1224
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Finnish
Swedish
Polish

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