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Affinity Coupling

 

G-Biosciences provide an array of agarose-based resins and magnetic beads with a variety of coupling chemistries for fast and reliable immobilization of functional groups.

Amine Magnetic Beads are 1µm, uniform magnetic beads with an amine (-NH2) surface functional group. Carboxyl Magnetic Beads are 1µm, uniform magnetic beads with a carboxyl (-COOH) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the primary amino group (-NH2) or carboxyl groups (-COOH) are joined to the magnetic beads through a short hydrophilic linker. The hydrophilic surface ensures the magnetic beads excellent dispersion ability and easy handling property in a wide variety of buffers. 

The Carbohydrate Coupling Resin (Hydrazide resin) is designed for the simple and efficient coupling of glycoproteins to a solid agarose support through oxidized sugar groups. The coupling is through the hydrazide group that is immobilized to agarose beads. The resin is ideal for immobilized polyclonal antibodies as these are abundant in carbohydrates in their Fc domain. The location in the Fc domain ensures the antibody’s binding site is orientated away from the resin for optimal binding and reduced steric hindrance. 

The formation of reactive imidazole carbamates is reliable and affordable with CDI Amine Reactive Agarose. CDI Amine Reactive Agarose is ideal for immobilizing peptides, small organic molecules and certain proteins and reactions can occur in organic solvent making it ideal for water-insoluble ligands.

CNBr-Activated Agarose is pre-activated high capacity resin used for covalent coupling of antibodies and large protein molecules for affinity chromatography. CNBr-Activated Agarose coupling offers several advantages. It is simple, reproducible, offer multi-point attachment of many protein ligands that results in chemically stable resin for affinity chromatography. CNBr-Activated Agarose is ideal for coupling of enzymes, antibodies and other proteins as the coupling is done under very mild conditions.

ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting in 15-atom hydrophilic spacer arm.

Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligands containing amine groups, thiol or hydroxyl groups.

The amine reactive HOOK™ Agarose Coupling Kit is designed for the simple and efficient coupling of proteins and peptides to a solid support. The kit allows the generation of both a stable and reusable column for the affinity purification of antibodies, proteins and other protein interacting biomolecules.

The amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by reductive amination, using sodium cyanoborohydride to form stable amine linkages between the agarose and the ligand. For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors, G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their primary amines.

For purification and calcium binding C-reactive proteins such as human and canine CRP we recommend Immobilized O-Phosphorylethanolamine.  Immobilized O-Phosphorylethanolamine is a more economical substitute for p-Amino Phosphoryl Choline Agarose.

NHS-Activated Agarose consists of 4% cross-linked agarose that has been activated by the addition of a reactive NHS (N-hydroxysuccinimide) group. The NHS group forms covalent, chemically stable amide bonds with ligands that contain primary amines. The NHS-Activated Agarose also contains a spacer arm between the NHS group and the agarose beads, making it suitable for coupling of small proteins and peptides. The long spacer arm minimizes steric hindrance allowing high efficient binding of ligands, including small proteins and peptides.

The SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification.  The kit uses our Immobilized DADPA (diaminodipropylamine) resin that can bind steroids, drugs and chemical compounds through their active hydrogens.

For affordable, efficient, and simple coupling of peptides and proteins to a 6% agarose support through free sulfhydryl groups (-SH).  The iodoacetyl groups of the Sulfhydryl Coupling Resin specifically react with free sulfhydryls to form covalent, permanent thioether bonds. The long spacer arm reduces steric hindrance and ensures greater binding of proteins and antibodies during affinity purification.  This resin is also available in a kit specifically optimized for immobilizing peptides, Sulfhydryl Immobilization Kit for Peptides.  Also available is a Sulfyhydryl Coupling Resin kit optimized for protein immobilization, Sulfhydryl Immobilization Kit for Proteins.

 

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

 

 

The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. This resin is also termed as EAH-Agarose.   Molecules, including proteins, peptides and nucleic acids, are c..
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G-Biosciences’ Carboxyl Magnetic Beads are 1µm, uniform magnetic beads with surface functional group -COOH. The magnetic bead consists of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the carboxyl groups (-COOH) are joined to..
$0.00
G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.    The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to preve..
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CNBr-Activated Agarose is pre-activated high capacity resin used for covalent coupling of antibodies and large protein molecules for affinity chromatography.    Cyanogen bromide reacts with hydroxyl groups of resin to form cyanate esters which inturn reacts with primary amine ligand..
$0.00
ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting in 15-atom hydrophilic spacer arm.    ..
$0.00
Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligands containing amine groups, thiol or hydroxyl groups.   Epoxide-Acivated Agarose is prepared by immobilization of ox..
$0.00
An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers a complete kit for the coupling of peptides and proteins to agarose through thei..
$0.00
NHS-Activated Agarose consists of 4% cross-linked agarose that has been activated by the addition of a reactive NHS (N-hydroxysuccinimide) group. The NHS group forms covalent, chemically stable amide bonds with ligands that contain primary amines. The NHS-Activated Agarose also contains a spacer arm..
$0.00
The SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification.  The ..
$0.00
The Sulfhydryl Immobilization Kit for Peptides is designed for the simple and efficient coupling of sulfhydryl-containing peptides to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent th..
$0.00
The Sulfhydryl Immobilization Kit for Proteins is a complete kit designed for the simple and efficient coupling of proteins to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent thioether..
$0.00
G-Biosciences’ Amine Magnetic Beads are 1µm, uniform magnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the primary amino group (-NH2) are..
$0.00
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