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Immobilized Iminobiotin Resin

Catalog Description Size Price(USD) Qty
786-599
Immobilized Iminobiotin 5ml resin
$121.00

Immobilized Iminobiotin Resin

Immobilized Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non-denaturing elution buffers.

 

The normal biotin-avidin complex requires strong denaturing buffers, i.e. 8M guanidine•HCl, to denature the avidin and release the biotin, which obviously destroys the native and functional aspects of the avidin.  The iminobiotin-avidin complex will form at >pH9.5 and can be dissociated at pH4.0 with gentle elution buffers, including 50mM ammonium acetate, pH4.0 with 0.5M NaCl.
 

 

Click here to request bulk or custom sizes.

 


Features

  • Biotin Binding Capacity: >2mg avidin/ml resin
  • No requirement for strong, denaturing elution buffers
  • Elutes at pH4.0

 


Applications

  • Isolation of avidin, streptavidin and Neutravidin complexes

 

 

Protocol
786-599
Material Safety Data Sheet
786-599
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Technical Literature
Protein Labeling & Conjugation
Protein Purification Handbook
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