Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein A/G binds well to IgG subclasses but does not bind IgA, IgM or serum albumin. This makes Protein A/G an excellent tool for purification and detection of monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and serum albumin. Individual subclasses of monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G.
The Immobilized Protein A/G 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. Each 1ml column enables purification of 1-13mg IgG from 0.5-2ml of serum or other sample.
The Immobilized Protein A/G 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples. Each 0.2ml column enables purification of 0.1-1mg IgG from 25-500µl of serum or other sample.
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- High binding capacity: 38mg human IgG/ml resin; >20mg sheep IgG/ml resin
- Ligand: Recombinant Streptococcal protein A/G lacking the albumin binding sites expressed in E. coli
- Bead size: 50-165μm
- Bead Structure: 6% highly cross-linked agarose
- Suitable for immunoaffinity chromatography and immunoprecipitation.