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G-Trap™ Octyl Agarose 6 Fast Flow

Catalog Description Size Price(USD) Qty
786-1003
G-Trap™ Octyl Agarose 6 Fast Flow, 1 ml 5 columns
$179.00
786-1004
G-Trap™ Octyl Agarose 6 Fast Flow, 5 ml 5 columns
$403.00

G-Trap™ Octyl Agarose 6 Fast Flow

G-Trap™ Octyl Agarose 6 Fast Flow are prepacked ready to use columns for seperation of biomolecules via Hydrophobic Interaction chromatography (HIC). 

 

Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of surface-exposed hydrophobic amino acids present on the protein or enzyme to be separated and the concentration and type of salt used in binding or start buffer. In HIC, the binding of biomolecules to the column (ligand) is facilitated by using higher concentrations of anti-chaotropic salts such as ammonium sulfate, sodium sulphate etc in the binding buffer or start buffer.

 

Straight alkyl chains ligands such as butyl, octyl show pure hydrophobic character whereas aryl ligands such as phenyl show mixed mode behavior where aromatic, hydrophobic as well as lack of charge play role in protein/biomolecules adsorption to the column.

 

G-Biosciences offer a wide range of G-Trap™ HIC Columns such as G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow , G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub), G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) so that one can choose the HIC column that is suitable for separation of the target protein. In addition G-Trap™ HIC Selection Kit is available for initial screening for appropriate hydrophobic column and purification conditions for the target protein

 

The properties of G-Trap™ Octyl Agarose 6 Fast Flow resin and column are listed below

 

G-Trap™ Octyl Agarose 6 Fast Flow Resin

Mean particle size: 90 µm

Bead structure: Highly cross-linked 6% Agarose

Ligand: Octyl

Ligand Concentration: About 5μmol/ml

Binding Capacity: About 30mg HSA/ml resin

pH stability Working Range: 3-13

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Pressure (MPa): 0.3

Maximum Flow Velocity: 450cm/h

Exclusion Limit (Globular Proteins): 4 x 106

Physical Stability: Negligible volume variation due to changes in pH or ionic strength

Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Storage Conditions: 4 to 30°C, 20% Ethanol

 

G-Trap™ Octyl Agarose 6 Fast Flow, 1 ml columns

Column Volume: 1 ml

Columns Dimension: 0.7 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

 

G-Trap™ Octyl Agarose 6 Fast Flow, 5 ml columns

Column Volume: 5 ml

Columns Dimension: 1.6 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

 

Application(s): 

  • G-Trap™ Octyl Agarose 6 Fast Flow are prepacked ready to use columns for separation of biomolecules via Hydrophobic Interaction chromatography (HIC).

 

Protocol
786-1003
786-1004
Material Safety Data Sheet
786-1003
English_US
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English_UK
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786-1004
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
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