A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, Aprotinin, Bestatin, E-64, EDTA, Leupeptin, Pepstatin A and PMSF that inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases. An EDTA solution is provided separate for enhanced metalloprotease inhibition. It is not present in the actual Mammalian ProteaseArrest™ cocktail as it would inhibit the activity of proteins that require divalent cations (Ca²+, Mg²+ or Mn²+) for their biological activity. In addition, EDTA will inhibit the purification of proteins using immobilized metal affinity chromatography.
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Sample Type & key features
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Inhibition Specificity
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1X Composition
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Concentration & Available Sizes
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For mammalian tissue and cell extracts
EDTA is supplied in a separate vial
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Mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases
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668µm AEBSF
0.3µM Aprotinin
3µM Bestatin
14µM E-64
5mM EDTA
5.25µM Leupeptin
1µM Pepstatin A
1mM PMSF
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100X DMSO solution
1ml, 5ml
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Features
- Broad spectrum, mammalian specific protease inhibitor cocktail.
- 100X concentrated, ready-to-use solution.
- Versatile as a separate vial of EDTA, a metalloprotease, is provided to protect proteins that require metal ions or if IMAC is a downstream application
- No proprietary ingredients, fully disclosed formulation of eight broad range protease inhibitors, including AEBSF, Aprotinin, Bestatin, E-64, EDTA, Leupeptin, Pepstain A and PMSF
Applications
- Inhibition of protease activity in protein preparations of mammalian lysates.
- Protection of proteins from proteolysis in such applications as electrophoresis, purification, storage, assays, and other applications.
ProteaseArrest Family
Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition. The ProteaseArrest™ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species. In addition to Mammalian, species-specific cocktails are available for Plant, Yeast, Fungus and Bacterial samples. Our Recom ProteaseArrest™ is designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. Recom ProteaseArrest™ contains a specific bacterial metalloproteases that does not interfere with 6X His protein purification. Our FOCUS™ ProteaseArrest™ is designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis. The TCM ProteaseARREST™ protease inhibitor cocktail is a broad range, 200X concentrated, ready-to-use protease inhibitor cocktail sterile filtered for tissue culture media. TCM ProteaseArrest™ inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases. Designed for the protection of proteins secreted in to the culture media.
