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Fractionation and Enrichment Kits

The analysis of a proteome is often inhibited by the vast amount of proteins, with large abundant proteins inhibiting the signal of lower abundance and often more interesting proteins.  Researchers overcome this problem by using fractionation, however inconsistencies in techniques and buffers often result in a lack of reproducibility.
G-Biosciences offers a wide selection of fractionation kits for processing samples from cells, tissues, bacteria, yeast, plants, and other types of samples. A selection of sample preparation accessories and supplies are also available. The following kits, accessories, and supplies are suitable for the analysis of proteins using electrophoresis and other biochemical techniques.

The FOCUS line of products allow for the fractionation of a large selection of biological samples into a multitude of different fractions and these resulting fractions are compatible with 2D electrophoresis and subsequent protein identification techniques.

Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT has been specifically developed for substantial removal of albumin from such samples.

We also offer a large variety of accessories to aid in protein fractionation and enrichment procedures.
 
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  • AlbuminOUT™

    >98% Removal of Albumin

      

  • FOCUS™ Soluble & Insoluble

    For the fractionation of soluble and insoluble proteins.

      

  • FOCUS™ Glycoprotein

    For the enrichment of glycoproteins

      

  • Nuclear & Cytoplasmic Extraction Kit

    For the enrichment of nuclei and cytoplasm.

      

  • FOCUS™ SubCell Kit

    For the fractionation of mitochondrial, nuclear, and cytosolic soluble and membrane prot.....

      

  • FOCUS™ PhosphoRich™

    For the enrichment of phosphopeptides and phosphoproteins.

      

  • FOCUS™ Global Fractionation

    Fractionation and enrichment of cytosolic and membrane signal, peripheral or integral pr.....

      

  • FOCUS™ Membrane Proteins

    For the fractionation of highly enriched membrane proteins

      

  • FOCUS™ Signal Proteins

    For the fractionation of signal proteins enriched in lipid rafts