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Human Cancer Cell Line Blots

Download the Protein Discovery Handbook for full list of lysates & blots

 

Blot is prepared using total protein extracted from a variety of human cancer cell lines. 
The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buff..
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