Biotin Labeling Kits

The HOOK™ Biotin kits are designed for the labeling, purification and quantification of proteins with biotin.

 

Protein Labeling

Each kit is supplied with a specific HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates.  The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.  The HOOK™ Biotin kits include EDC as the carbodiimide crosslinker in the carboxyl coupling kits and sodium meta-periodate for carbohydrate oxidation in the carbohydrate coupling kits.

In addition to the above, each HOOK™ Biotin kit contains a specific Optimizer Buffer that provides the optimal reaction conditions for each HOOK™ Biotin Reagent.

 

Purification

Following the labeling of the protein with the HOOK™ Biotin Reagent the unreacted biotin and other chemicals are rapidly removed from the labeled protein with the supplied SpinOUT™ Columns. These columns use gel filtration to remove the by-products in <10 minutes.

 

Estimation of Biotin Incorporation

Following the purification of the labeled protein, the HOOK™ Biotin kits contain the reagents to estimate biotin incorporation using the HABA and avidin assay. The assay can be performed in <5 minutes.

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HOOK™ IgG Biotinyaltion (Sulfhydryl)

Simple Antibody Biotinylation   The HOOK™ IgG Biotinylation (Sulfhydryl) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid supp..

HOOK™ IgG Biotinylation (Amine)

Simple Antibody Biotinylation   The HOOK™ IgG Biotinylation (Amine) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid support (..

HOOK™-Biotin-BMMCC Labeling Kit

HOOK™-Biotin-BMMCC reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of..

HOOK™-Biotin-Hydrazide Labeling Kit

HOOK™-Biotin-Hydrazide reacts with oxidized carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself but conjugate to the carbohydrate residues of glycoproteins...

HOOK™-Biotin-LC-Hydrazide Labeling Kit

HOOK™-Biotin-LC-Hydrazide reacts with oxidized carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself but conjugate to the carbohydrate residues of glycoprotei..

HOOK™-Biotin-PDA Labeling Kit

HOOK™-Biotin-PDA reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side chain of t..

HOOK™-Biotin-PEG2-Amine Labeling Kitn

HOOK™-Biotin-PEG2-Amine reacts with protein carboxyl groups in the presence of the heterobifunctional cross-linker EDC. HOOK™-Biotin-Pentylamine, HOOK™-Biotin-PEG2-Amine and HOOK&tra..

HOOK™-Biotin-PEG3-Amine Labeling Kit

HOOK™-Biotin-PEG3-Amine reacts with protein carboxyl groups in the presence of the heterobifunctional cross-linker EDC. HOOK™-Biotin-Pentylamine, HOOK™-Biotin-PEG2-Amine and HOOK&tra..

HOOK™-Iodoacetyl-LC-Biotin Labeling Kit

HOOK™-Iodoacetyl-LC-Biotin reacts with protein free sulhydryl groups. Sulfhydryl reactive reagents are more specific and react only with free sulfhydryl residues (-SH or thiol groups). The side ..

HOOK™-NHS-Biotin Labeling Kit

HOOK™ NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common..

HOOK™-NHS-dPEG™4-Biotin (Micro)

HOOK™-NHS-dPEG™4-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and repres..

HOOK™-NHS-dPEG™4-Biotin Labeling Kit

HOOK™-NHS-dPEG™4-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and repres..