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GST-Tagged Protein Purification

 

G-Biosciences provide several reagents for affinity purification of glutathione S-transferase (GST) tagged proteins. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~40mg GST/ml resin. Supplied as slurry in 20% ethanol. Glutathione Resin is available as resin alone or supplied in a kit format. Binding/ Wash and Elution buffers are available, in addition to reduced glutathione for purification.

For the purification of soluble GST-tagged proteins from bacterial cultures we provide HOOK™ GST Protein Purification Kit (Bacteria). The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by passing clarified lysate through prepacked columns.

For the purification of soluble GST-tagged proteins from yeast cultures we provide HOOK™ GST Protein Purification Kit (Yeast). The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by passing the clarified lysate through prepacked columns.

Rapid purification of soluble GST-tagged proteins from Bacteria or Yeast is simple and affordable with a HOOK™ GST Protein Spin Purification Kit (Bacteria) and the HOOK™ GST Protein Spin Purification Kit (Yeast). After lysis and release of the solubilized protein, the GST tagged protein is purified by affinity chromatography by adding 0.5ml glutathione resin to the clarified lysate. The resin is transferred to a convenient spin column, where it is rapidly washed and the GST protein is eluted.

 

Download the Purification Resins Handbook for Complete Product ListDownload the Protein Purification Handbook for a full list of related products

Synonym: γ-L-Glutamyl-L-cysteinyl-glycine, GSH   Amino Acid Sequence: γ-Glu-Cys-Gly   CAS Number: 70-18-8   Linear Formula: C10H17N3O6S   Molecular Weight: 307.32   Solubility: Water (50 mg/ml). Reduced glutathione is freely soluble in water, dilute alc..
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Features Glutathione resin also available    ..
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Features Supplied with dry glutathione to limit oxidation Glutathione resin also available..
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HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from bacterial cultures.  The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity of the..
$0.00
HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of the pr..
$0.00
HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activ..
$0.00
HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activit..
$0.00
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin. Su..
$0.00
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