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Agarose based affinity chromatography resins with immobilized lectins for the purification of glycoproteins.
Immobilized Jacalin: Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa. Jacalin is a α-D-galactose binding lectin purified from jack-fruit (Artocarpus integrifolia) seeds. Applications include isolating IgA from human serum and colostrums, isolating human plasma glycoproteins and histochemistry. Jacalin also binds IgD.
Concanavalin A (Con A) Agarose: Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. Con A agarose has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles.