Biotin-Tagged Purification

 Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1) and streptavidin (Ka=1015 M-1). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by boiling in SDS Page Sample Loading Buffer.

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HOOK™ BiotinQuant

HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-..

HOOK™ Cell Surface Protein Isolation

HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to convenient..

Streptavidin Binding Buffer

Features 20mM NaPO4, 0.15M NaCl (pH 7.5) Streptavidin resin also available ..

Streptavidin Elution Buffer

Features 8M Guanidine.HCl, pH 1.5 Streptavidin resin also available ..

Immobilized Avidin Resin

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can surviv..

Immobilized Monomeric Avidin Resin

G-Biosciences Immobilized Monomeric Avidin Resin is designed for the simple affinity chromatography purifications of proteins, antibodies and other molecules with a biotin tag.  The resin consist..

Immobilized Streptavidin Resin

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin a..