Biotin-Tagged Purification

 Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1) and streptavidin (Ka=1015 M-1). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by boiling in SDS Page Sample Loading Buffer.

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Streptavidin Binding Buffer

Features 20mM NaPO4, 0.15M NaCl (pH 7.5) Streptavidin resin also available ..

HOOK™ BiotinQuant™

HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-..

HOOK™ Cell Surface Protein Isolation

HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to convenient..

Immobilized Avidin Resin

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can surviv..

Immobilized Monomeric Avidin Resin

G-Biosciences Immobilized Monomeric Avidin Resin is designed for the simple affinity chromatography purifications of proteins, antibodies and other molecules with a biotin tag.  The resin consist..

Immobilized Streptavidin Resin

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹âµM-¹) and streptavidin (Ka=10¹âµM-¹). Biotin a..

Streptavidin Elution Buffer

Features 8M Guanidine.HCl, pH 1.5 Streptavidin resin also available     ..