Immobilized Heparin is a ready-to-use purification resin for a wide range of proteins. The resin consists of 4% cross-linked agarose covalently coupled to heparin through amide bonds. The coupling chemistry used generates a highly stable purification resin that is stable most commonly used buffers and denaturants.
Heparin is a linear glycosaminoglycan composed of equimolar quantites of glucosamine and glucuronic acid, alternatively linked by α(1→4) glycosidic bonds. A number of its hydroxyl groups are esterified with sulfuric acid moieties and the molecule has a single reducing sugar terminus.
Due to its structure and biochemical role, Heparin is able to bind a number of proteins, enzymes and polycationic organic compounds. The binding is either ionically or more specific protein-ligand or enzyme-inhibitor (or enzyme-activator) interactions.
Several classes of proteins can bind to heparin, including:
- Coagulation Factors: ATIII, Factors IX, VII, XI, XII and XIIa
- Lipoprotein Lipases: By ionic interactions
- Lipoproteins: LDL, VLDL, VLDL apoprotein, HDL
- Growth Hormones
- Growth Factors: FGF, ECGF
- DNA- & RNA- Related Enzymes
- Enzymes: Collagenase, hyaluronidase, lysozyme, proteases