Immobilized Heparin

Immobilized Heparin is a ready-to-use purification resin for a wide range of proteins.  The resin consists of 4% cross-linked agarose covalently coupled to heparin through amide bonds.  The coupling chemistry used generates a highly stable purification resin that is stable most commonly used buffers and denaturants.

 Heparin is a linear glycosaminoglycan composed of equimolar quantites of glucosamine and glucuronic acid, alternatively linked by α(1→4) glycosidic bonds.  A number of its hydroxyl groups are esterified with sulfuric acid moieties and the molecule has a single reducing sugar terminus.  

 Due to its structure and biochemical role, Heparin is able to bind a number of proteins, enzymes and polycationic organic compounds.  The binding is either ionically or more specific protein-ligand or enzyme-inhibitor (or enzyme-activator) interactions.

Several classes of proteins can bind to heparin, including:

  • Coagulation Factors: ATIII, Factors IX, VII, XI, XII and XIIa
  • Lipoprotein Lipases: By ionic interactions
  • Lipoproteins: LDL, VLDL, VLDL apoprotein, HDL
  • Growth Hormones
  • Growth Factors: FGF, ECGF
  • DNA- & RNA- Related Enzymes
  • Enzymes: Collagenase, hyaluronidase, lysozyme, proteases
 
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Immobilized Heparin

G-Biosciences Immobilized Heparin is a ready-to-use purification resin for a wide range of proteins.  The resin consists of 6% cross-linked agarose covalently coupled to heparin through amide bon..