Histidine-Tagged Protein Purification

Immobilized metal affinity chromatography resins for 6X His tagged protein purification

A large selection of resins and kits for the isolation of His tagged recombinant proteins are available.

Four different IMAC purification resins are offered:

Nickel Chelating Resin: The most commonly used IMAC purification resin for the purification of 6X His recombinant proteins that offers high binding efficiency and low non-specific binding. 

 

Cobalt Chelating Resin: Growing increasingly popular due to its advantage over Nickel Chelating Resin.  Although 6X His recombinant proteins bind with a slightly lower efficiency compared to Nickel Chelating Resin there is a significant reduction in non-specific binding. Cobalt resins have a higher selectivity for poly-His sequences, however have a low loading capacity, therefore Cobalt Chelating Resin should be used for valuable recombinant proteins in limited quantities. 

 

Zinc Chelating Resin: For the purification on zinc binding proteins, including 6x His recombinant proteins. Available as a resin.

 

Copper Chelating Resin: For the purification on copper binding proteins, including 6x His recombinant proteins. Available as a resin.

Cobalt has the highest selectivity of the resins followed by Zinc, Nickel then Copper, but has the lowest loading capacity.  Copper has the highest loading capacity, followed by Nickel then Zinc.

Show:
Sort By:

Co-NTA Resin

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with c..

G-Sep™ Co IDA Agarose Fast Flow

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with c..

G-Sep™ Ni IDA Agarose Fast Flow

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with c..

His Binding/ Wash Buffer

His Binding/ Wash Buffer for Immobilized Metal Affinity Chromatography. Suitable for our Immobilized Cobalt, Copper, Nickel, and Zinc chelating resinsFeatures 10mM Imidazole, 300mM NaCl, 50mM so..

His Elution Buffer

His Elution Buffer for Immobilized Metal Affinity Chromatography.   Suitable for our Immobilized Cobalt, Copper, Nickel, and Zinc chelating resinsFeatures 250mM Imidazole, 300mM NaCl, 50mM..

HOOK™ 6X His Protein Purification (Bacteria)

HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from bacterial cultures.  The bacteria are first lysed with Bacterial PE LB™ and PE..

HOOK™ 6X His Protein Purification (Yeast)

HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from yeast cultures.  The yeast are first lysed with Yeast PE LB™ and LongLife&trad..

HOOK™ 6X His Protein Spin Purification (Bacteria)

HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from bacterial cultures.    The bacteria are first lysed with Bacterial..

HOOK™ 6X His Protein Spin Purification (Yeast)

HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from yeast cultures.  The yeast are first lysed with Yeast PE LB™ and Lo..

Ni-NTA Magnetic Beads

G-Biosciences’ Ni-NTA Magnetic Beads are 3µm beads designed for the rapid purification of X His-tagged proteins. Ni-NTA Magnetic Beads have nitrilotriacetic acid (NTA) groups with charged ..

Nickel Chelating Resin

Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag ..

Cobalt Chelating Resin

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cati..