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Histidine-Tagged Protein Purification

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

 

Immobilized metal affinity chromatography resins for 6X His tagged protein purification

A large selection of resins and kits for the isolation of His tagged recombinant proteins are available.

Four different IMAC purification resins are offered:

Nickel Chelating Resin: The most commonly used IMAC purification resin for the purification of 6X His recombinant proteins that offers high binding efficiency and low non-specific binding. 

 

Cobalt Chelating Resin: Growing increasingly popular due to its advantage over Nickel Chelating Resin.  Although 6X His recombinant proteins bind with a slightly lower efficiency compared to Nickel Chelating Resin there is a significant reduction in non-specific binding. Cobalt resins have a higher selectivity for poly-His sequences, however have a low loading capacity, therefore Cobalt Chelating Resin should be used for valuable recombinant proteins in limited quantities. 

 

Zinc Chelating Resin: For the purification on zinc binding proteins, including 6x His recombinant proteins. Available as a resin.

 

Copper Chelating Resin: For the purification on copper binding proteins, including 6x His recombinant proteins. Available as a resin.

Cobalt has the highest selectivity of the resins followed by Zinc, Nickel then Copper, but has the lowest loading capacity.  Copper has the highest loading capacity, followed by Nickel then Zinc.

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.     The Co-NTA Resin is specifically designed for the purif..
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.    The Cobalt Chelating Resin is specifically designed for the..
$0.00
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  The Copper Chelating Resin is specifically designed for the pu..
$0.00
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™ Co IDA Agarose Fast Flow is specifically designed fo..
$0.00
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™ Ni IDA Agarose Fast Flow is specifically designed fo..
$0.00
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™NI NTA Agarose Fast Flow is specifically designe..
$0.00
G-Trap™ Ni IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).   IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein resid..
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His Binding/Wash Buffer for Immobilized Metal Affinity Chromatography.   Suitable for our Immobilized Cobalt, Copper, Nickel, and Zinc chelating resins   Features 10mM Imidazole, 300mM NaCl, 50mM sodium phosphate (pH8.0) Immobilized Cobalt, Copper, Nickel, and Zinc chelating resin..
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His Elution Buffer for Immobilized Metal Affinity Chromatography.   Suitable for our Immobilized Cobalt, Copper, Nickel, and Zinc chelating resins   Features 250mM Imidazole, 300mM NaCl, 50mM sodium phosphate (pH8.0) Immobilized Cobalt, Copper, Nickel, and Zinc chelating resins ar..
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HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity o..
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HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of ..
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HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from bacterial cultures.    The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the str..
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